Clone

Target

IDP95258  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Oct 02, 2014  

Experiment End Date

Oct 02, 2014  

Person

Brian Bishop  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

667  

Reference Sequence Used

MVAIPSGNTI KLVPDSNVKN SGIPYDSRNR VRGDQIVTRV IWLENTNPND LIPALRPLMP QFAHMAAIAG TNALIISDRA ANIYQLENII RNLDGTGQND IEAITLQSSQ AEEIITQLEA MSATGASKDF SGARIRIIAD NRTNRILIKG DPQTRKRIRH MIEMLDVPSA DRLGGLKVFR LKYASAKNLS EILQGLVTGQ AVSSSNNSNN SSNSSNPINS LIGNNQNSGS NTSGSNGASI STPAINLNGN SNSSNQNNIT SFNQNGVSII ADNAQNSLVV KADPQLMREI ESAIQQLDVR RQQVLIEAAI IEVSGDDADQ LGIQWALGDL SSGIGLLSFS NVGASLSSIA AGYLSGGSAG AASAIANGAN KGNGATLGLG NFDNSRKAYG ALIQALKTNT KSNLLSTPSI VTMDNEEAYI VVGQNVPFVT GSVTTNSTGI NPYTTVERKD VGVTLKVVPH IGEGGTVRLE VEQEVSAVQD SRGQAADLVT SKRAIKTAVL AEHGQTVVLG GLVSDDTSLS RQGIPGLSSI PYVGRLFRSD NRSNVKRNLL VFIHPTIVGD ANDVRRLSQQ RYNQLYSLQL AMDKNGNFAK LPEQVDDIYN QKMTPPSIAS KPKNYQQVPS GGKSSTITTP VAVEPTVQKQ TLQLPDPEIN RTKNTVTTTT LRPSTAP  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTTGCAATTCCGTCTGG  

PCR Backward Primer

TTATCCACTTCCAATGCTACGGCGCTGTGCTTGG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014