Clone

Target

IDP95259  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 19, 2014  

Experiment End Date

Sep 23, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

361  

Reference Sequence Used

MARASEALEH LDVAPGLEEL EMGAQRVQVD DKAMINCRAD LNQLVPFKYE WAWQKYLDGC ANHWMPQEVN MNHDIALWKS ENGLTEDERT IVMRSLGFFS TADSLVANNL VLAIYRHITN PECRQYILRQ AFEEAIHTHA YQYCIESLGM DEGEVFNMYR EIPSVARKAA WGLKYTQSLS DPTFHTGTPE NDQRLLRNLI AFYCVLEGIF FYCGFTQILS MGRRNKMNGV AEQFQYILRD ESMHLNFGID MINQIKIENP HLWTAEFQQE VIQMILEGTM LEIEYARDTM PRGVLGMNAS MMEEYLKFIC NRRLSQLGLP EQFAGVTNPF AWMSEMMDLR KEKNFFETRV TDYQTGGALS W  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MARASEALEH LDVAPGLEEL EMGAQRVQVD DKAMINCRAD LNQLVPFKYE WAWQKYLDGC ANHWMPQEVN MNHDIALWKS ENGLTEDERT IVMRSLGFFS TADSLVANNL VLAIYRHITN PECRQYILRQ AFEEAIHTHA YQYCIESLGM DEGEVFNMYR EIPSVARKAA WGLKYTQSLS DPTFHTGTPE NDQRLLRNLI AFYCVLEGIF FYCGFTQILS MGRRNKMNGV AEQFQYILRD ESMHLNFGID MINQIKIENP HLWTAEFQQE VIQMILEGTM LEIEYARDTM PRGVLGMNAS MMEEYLKFIC NRRLSQLGLP EQFAGVTNPF AWMSEMMDLR KEKNFFETRV TDYQTGGALS W  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCTCGAGCATCTGAAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTACCAGCTTAACGCACCAC  

Shipped To Lab

ANL  

Shipped Date

Jan 05, 2015  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014