Clone

Target

IDP95260  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 19, 2014  

Experiment End Date

Sep 23, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

516  

Reference Sequence Used

MQPASSEAAV VRNYLEVILD TPWNKASKVS INLNKAQEIL DADHYGLDDV KDRIVEYLAV QSRVKKLKGP ILCLVGPPGV GKTSLGESVA KATGREFVRM ALGGVRDEAE IRGHRRTYIG AMPGKIVQSL TKVGVKNPLF LLDEIDKMAQ DYRGDPASAL LEVLDPSQNS KFNDHYLDLD LDLSEVMFIC TANSMNIPEA LLDRMEVIRL PGYTEDEKVN IAERYLVPKA IKNNGLRPKE LTIHEEAIRD IVQRYTREAG VRNLEREVSK IARKVVKEAV SKKSKNLQLD VTSANLPEYL GPHKFDFGMA EDEAQVGRVN GLAWTSVGGE LLTIEVAAVK GKGKFITTGS LGDVMKESIT TAMTVVRTRA DELGIEASRF EETDVHVHLP EGATPKDGPS AGLALTTALV SAFTGIAIRP DIAMTGETSL GGRAMRIGGL KEKLLAAHRG GIKLVFIPQD NVRDLAEIPD NVKEGLEIKA VKSIDEILPL ALTSMPKPLP KTPIVKPVEG SKAARH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAGCCTGCATCTAGTGA  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTGACGCGCTGCTTTTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014