Clone

Target

IDP95265  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 19, 2014  

Experiment End Date

Sep 23, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

417  

Reference Sequence Used

MIAVMALVMG LFTAFQTWIY RRFFPETPLT FAPLWIIFEW AKTWVFTGFP WLFVGYAFTE RLLDGYAPLF GIYAISFVVI VLACALVEVL RKRIFWVIPA ALLVLGAWGA SYIQFVQPKA AKPLSVSLIQ GNIPQDLKWL TEYQVRTLEI YAGLTQSEWG RDLIVWPESS IPLFQTDIEP FLDAMDAQAK KNHTAWVTGI PYWDVAKSHQ VGSPLYYNSI MASGSDSSGL YKKQRLVPFG EYIPLSGLLS WVLPAMQNDI SMSGFTRGES DQKPLLIKGH ALAAAICYEV AYPNLTRRNA EDSDFLVTVS NDAWFTGTAG PWQHLQMVQM RAKENGRWFI RATNTGITAF IDQNGHITEQ APIDKEFVLR GDLPAMQGQT LYNRLGDYPI LGFAILLLVL GWIYRPRKVD VSYKSRR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTGCTGTTATGGCCCTT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCGACGTGATTTGTAAGAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014