Clone

Target

IDP95269  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Oct 02, 2014  

Experiment End Date

Oct 02, 2014  

Person

Brian Bishop  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

327  

Reference Sequence Used

MTRPDSKSMN YQLLKTFSRQ PIQFGRFLAR LLAGLVNTLK ITRTSKSIEL NLRIALPYLT PQQRIAITEK AVRNELTSYF EFLSIWGSSN SKNISRIHRI EGEHFFHEAL AAKKGVVLIV PHFGTWEVMN AWCAQFTSMT ILYKPVKNAD ADRFVREARS REQANLVPTD ESGVRQIFKA LKQGETTVIL PDHTPNVGGD MVNYFGVPLA SSNLSAKLIQ KTKAKALFLY AIRNENDGFT MHIEPMDEKI YEGTADDGTY VIHQAIEQLI YQYPEHYHWS YKRFKANPAL DNIYNIDPTE ALKIVDRLKA EALKTSTQPE PIQTSVM  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACTAGACCAGATTCAAAAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACATGACCGACGTTTGGAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014