Clone

Target

IDP95270  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 19, 2014  

Experiment End Date

Sep 23, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

310  

Reference Sequence Used

MIAHPCYVDK FSQQVHFHAI DFARSRWNIF LLWQLKNLIQ QIQPAIVHAQ AGKAAELIAR IKPFLSGPKF ITTVHGTKKN KSAYLAGDAV IAVSQALTQG IPESKAHVVY NGVYPQPVLT TENKEKLLQS IQKDFTELDT SKRVVMCIGR LEPVKNISLL IESMQQIDAN LWIVGDGSLR ASLEKQVSEL NMQNRVAFLG FRTDARDLVQ LADIVVLSSD REGFPLVMVE ALQADKAMAS TKVNGVIEWL PEQYLAEIGD TQGLAKAIEY ALQPEAQNDF GPLYAKAKAE LTVPAMAEQT LNIYKGLLKT  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTGCACATCCTTGCTATG  

PCR Backward Primer

TTATCCACTTCCAATGTTAAGTTTTCAGCAGGCCTTTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014