Clone

Target

IDP00259  

Clone Suffix

302

Sequence Comments

MET and GLY at start replace signal peptide for expression in E.coli. Two mutations were required for crystal formation. Those were Y24 to D and F95 to D.  

Experiment Start Date

Jun 15, 2010  

Experiment End Date

Jun 30, 2015  

Person

Chris Nelson  

Lab

Washington University  

Protocol ID

WU_basic_cloning_protocol_1  

Status

success  

Vector

pET21A(+)  

Sequence Start

20  

Sequence End

168  

Reference Sequence Used

MGHKLAFNFN LEINGSDTHS TVDVYLDDSQ IITFDGKDIR PTIPFMIGDE IFLPFYKNVF SEFFSLFRRV PTSTPYEDLT YFYECDYTDN KSTFDQFYLY NGEEYTVKTQ EATNKNMWLT TSEFRLKKWF DGEDCIMHLR SLVRKMEDSK RNTG  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

 

PCR Backward Primer

 

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 23, 2015