Clone

Target

IDP95461  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Mar 30, 2015  

Experiment End Date

Mar 30, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

254  

Reference Sequence Used

MNNQWGLYST DYSFLKVLLR MLIRKEMVSL IPNILIVDDD PHIRELVSVF LECEGFQTYE AIDGLDALQK INQVKVDMVI LDIMMPNMDG FDVCFELRKY YDIPILILTA KGETSQKVKG FHLGTDDYLV KPFDPIELVV RVKALLKRYQ ITVSQSIQVG TVLLNRKTFE VTIGEQTVTL PLKEFELLFT LGSKAGRTCS REQLIEDVWG YDFEGNERTL DVHINRLREK FQEEKSKFSI KTIRGLGYRL EVSK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATAATCAGTGGGGATTGT  

PCR Backward Primer

TTATCCACTTCCAATGTCATTTACTTACCTCTAAGCGA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 18, 2015