Clone

Target

IDP95462  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Mar 30, 2015  

Experiment End Date

Mar 30, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

457  

Reference Sequence Used

MFKTLYARIA IYSITVILFS ALISFVLTNV YYHYNLKASN DAKIMKTLKE ARQYEQSAKP THIQQYFKHL GQMNYQIMTI DQKGHKTFYG EPFREDTLSQ NAINNVLNNQ DYHGIKDKPF ALFVTGFFDN VTDNTVGINF KTKDGSIAVF MRPDIGETFS EFRTFLAVLL MLLLFISISL VIASTYSIIR PVKKLKLATE RLIDGDFETP IKQTRKDEIG TLQYHFNKMR ESLGQVDQMR QHFVQNVSHE IKTPLTHIHH LLSELQQTSD KTLRQQYIND IYTITTQLSG LTTELLLLSE LDNHQHLLFD DKIQVNQLIK DIIRHEQFAA DEKSLIILAD LESINFLGNQ RLLHQALSNL LINAIKYTDV GGAIDIALQH SHNNIIFTIS NDGSPISPQA EARLFERFYK VSKHDNSNGL GLAITKSIIE LHHGTIQFTQ SNEYVTTFTI TLPNNSL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTAAAACACTCTATGCTAG  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAGTGAATTATTTGGCAGTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 18, 2015