Clone

Target

IDP95473  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Mar 30, 2015  

Experiment End Date

Mar 30, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

234  

Reference Sequence Used

MKFKEIEGST MEREWELFLA PYEQAVSELK VKLRGIRKQF REQDKHIPIE FVTGRVKPVD SILTKTAIRH IPLNRIEEEM QDIAGLRIMC QFVEDIYQVV ALLRNRKDLK IVEERDYIEN KKESGYRSYH VVVEYPVQLV TGEKIILAEI QIRTLSMNFW ATIEHSLNYK YQGVFPEEMK ERLQRAAEAA YLLDEEMSSI REEIQEAQHY FSHGRGLLEN EYYKQIKNET PENQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAATTCAAGGAAATTGAGGG  

PCR Backward Primer

TTATCCACTTCCAATGTTACTGATTTTCTGGTGTTTCG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 18, 2015