Clone

Target

IDP95475  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Mar 30, 2015  

Experiment End Date

Mar 30, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

221  

Reference Sequence Used

MNWEEFLDPY IQAVGELKIK FRGIRKQFRK QKRHSPIEFV TGRVKPIESI KEKMVLRGIK KENLTQDMQD IAGLRIMVQF VDDVNDVLEL LRQRKDMKVI QERDYINNLK PSGYRSYHVI VEYPVDTISG QRIIMAEIQI RTLAMNFWAT IEHSLNYKYH GEFPEDIKRR LELTSKIAFQ LDEEMRQIRD DIKEAQLLFD AETRKLNDGV GNSDDTDELY R  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNWEEFLDPY IQAVGELKIK FRGIRKQFRK QKRHSPIEFV TGRVKPIESI KEKMVLRGIK KENLTQDMQD IAGLRIMVQF VDDVNDVLEL LRQRKDMKVI QERDYINNLK PSGYRSYHVI VEYPVDTISG QRIIMAEIQI RTLAMNFWAT IEHSLNYKYH GEFPEDIKRR LELTSKIAFQ LDEEMRQIRD DIKEAQLLFD AETRKLNDGV GNSDDTDELY R  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATTGGGAAGAATTTCTGG  

PCR Backward Primer

TTATCCACTTCCAATGTTATCGGTAAAGTTCATCTGTG  

Shipped To Lab

ANL  

Shipped Date

Jun 17, 2015  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 18, 2015