Clone

Target

IDP95480  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 09, 2015  

Experiment End Date

Jun 09, 2015  

Person

Brian Bishop  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

510  

Sequence End

1114  

Reference Sequence Used

DNKKIPILTP NSTKIIECLE NLSNENSGSP IQLNDDILRN EEIFKVYISE IEKESETLYY LLKQHRYNYF NRVNQLVSQF LEISNYLQSN INKEHRCEIL EFIIEELNLW LFYSRFSISI SRTTFLLNSI SLPMPSFNNE FCSRFSENDS FNSNNKDGNS KGIIGFSHSD YIMDKGLCSL NNCQFLRIIP NECKIYNSRK RVHFLLAIEI ADLDDQDYEL GKNSQDLISK DILNYLKNHG IKLKTDNNDE NQNRSQDILL NFLNILSVGI SESIDMGLSQ EDFNQITFID NKDSDEIEDS SIHDNELSDE DIFINKDWPQ ETWSSKIKRY RKESPYSKLK SWGIKTLLLK SSDDLRQEYL ASQLLQQFDW IFKINKLPLW LHPYEILVIG SHGGFIEYIQ DTFSIDSLKR RFQTDNLLNC FDQLFKDEEL KSKARKCFVE SHAAYSLVSY FLQVKDRHNG NFLIDKHGHI IQIDYGFMLS NSPGNVNFEQ SPFKLTQEFL DVMMGENSSD FQYFQQLIIK GFLASRKHVD QIVLTIESMT SASKLPCFSL FTNKEYFIQD IRDRFFLHLT EEQCVVKVTE LIQSSINNWR SIQYDAFQRI TNGIL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGACAATAAGAAAATTCCGATCC  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGAATGCCGTTCGTGAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 12, 2015