Clone

Target

IDP00006  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Aug 05, 2015  

Experiment End Date

Aug 13, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

300  

Sequence End

442  

Reference Sequence Used

NQNEPDGVID HLMVIHAGVG QEAGGGKLGD DAIWSHRSKL AIDPVAIEGT KSKVDYFGGK VAAHDYTIEP EDGAVGVFAH EFGHDLGLPD EYDTKYTGTG SPVEAWSLMS GGSWTGKIAG TEPTSFSPQN KDFLQKNMGG NWA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGAATCAAAATGAACCTGATGGTG  

PCR Backward Primer

TTATCCACTTCCAATGTTATGCCCAGTTGCCACCC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 18, 2015