Clone

Target
IDP00009  
Clone Suffix
3
Experiment Start Date
Aug 05, 2015  
Experiment End Date
Aug 13, 2015  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
173  
Sequence End
363  
Reference Sequence Used
LPEKEWRSGY AEVIKHALIG DVKLYHWLKE EVQTLADLRD EKLIHILMKA IPVKANIVAQ DETEKGVRAH LNFGHTLGHA LEKELGYGNI THGDGVAVGM LFAIFLSEQV YKVNLAYEEM KQWFLNYGYP KMPSDLSVER LVGLMKQDKK ANAGTIHMVL MQEYGVVNVV SIPDETVHIA LEAFQKDMVE K  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGCTTCCTGAAAAAGAGTGGCG  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTTCAACCATATCCTTTTG  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2015-09-15 2015-09-15 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2015-10-19 2015-10-19 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay