Clone

Target

IDP95480  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Sep 04, 2015  

Experiment End Date

Sep 04, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pCPD  

Sequence Start

510  

Sequence End

1114  

Reference Sequence Used

MDNKKIPILT PNSTKIIECL ENLSNENSGS PIQLNDDILR NEEIFKVYIS EIEKESETLY YLLKQHRYNY FNRVNQLVSQ FLEISNYLQS NINKEHRCEI LEFIIEELNL WLFYSRFSIS ISRTTFLLNS ISLPMPSFNN EFCSRFSEND SFNSNNKDGN SKGIIGFSHS DYIMDKGLCS LNNCQFLRII PNECKIYNSR KRVHFLLAIE IADLDDQDYE LGKNSQDLIS KDILNYLKNH GIKLKTDNND ENQNRSQDIL LNFLNILSVG ISESIDMGLS QEDFNQITFI DNKDSDEIED SSIHDNELSD EDIFINKDWP QETWSSKIKR YRKESPYSKL KSWGIKTLLL KSSDDLRQEY LASQLLQQFD WIFKINKLPL WLHPYEILVI GSHGGFIEYI QDTFSIDSLK RRFQTDNLLN CFDQLFKDEE LKSKARKCFV ESHAAYSLVS YFLQVKDRHN GNFLIDKHGH IIQIDYGFML SNSPGNVNFE QSPFKLTQEF LDVMMGENSS DFQYFQQLII KGFLASRKHV DQIVLTIESM TSASKLPCFS LFTNKEYFIQ DIRDRFFLHL TEEQCVVKVT ELIQSSINNW RSIQYDAFQR ITNGIL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGGACAATAAGAAAATTCCGAT  

PCR Backward Primer

TGCTGCCCCCAGAATGCCGTTCGTGATGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 30, 2015