Clone

Target

IDP95480  

Clone Suffix

6

Sequence Comments

 

Experiment Start Date

Sep 23, 2015  

Experiment End Date

Sep 23, 2015  

Person

Brian Bishop  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

76  

Sequence End

1114  

Reference Sequence Used

GSLLRLFQSD VFDAHLHLYY IYHHKEFGVH EYLVNLLYER SDEILWYLPQ LCEISLNRFE KSSLWKYFLD KSSENMNFSL ILSWMYQAMN EDNIPLISEY AQNMLQEIEM AMVNSKLKPD IRYIEKRLYF WRRSNLSVFQ SLCNQNMKLN DIEDEFKDND NTMLMNRENT NTQLSLAIYS TLPSSETINL NESDDLNQDD NNRIISSDIN TNTIPNNNSK TIKLSSCDVL PDIWIYRIIN DDFSCENKAK IEYIQTKLGL INRQFKNLYS NATCAAYYNP NPGTSLNYKS GVSSSAFGSL NSGFLPTSII SGSSSFGTFS SGGNSNKVSI PYFFFSKLGS PFDLIDVNKI INDIQFRSKS RINLINKSHN FNVQDNSSED TVSIPKTPRD VIRITHPFSP CSETESETNN LDLDDNGTFE EIKLAPDSNV CSDNDNKKIP ILTPNSTKII ECLENLSNEN SGSPIQLNDD ILRNEEIFKV YISEIEKESE TLYYLLKQHR YNYFNRVNQL VSQFLEISNY LQSNINKEHR CEILEFIIEE LNLWLFYSRF SISISRTTFL LNSISLPMPS FNNEFCSRFS ENDSFNSNNK DGNSKGIIGF SHSDYIMDKG LCSLNNCQFL RIIPNECKIY NSRKRVHFLL AIEIADLDDQ DYELGKNSQD LISKDILNYL KNHGIKLKTD NNDENQNRSQ DILLNFLNIL SVGISESIDM GLSQEDFNQI TFIDNKDSDE IEDSSIHDNE LSDEDIFINK DWPQETWSSK IKRYRKESPY SKLKSWGIKT LLLKSSDDLR QEYLASQLLQ QFDWIFKINK LPLWLHPYEI LVIGSHGGFI EYIQDTFSID SLKRRFQTDN LLNCFDQLFK DEELKSKARK CFVESHAAYS LVSYFLQVKD RHNGNFLIDK HGHIIQIDYG FMLSNSPGNV NFEQSPFKLT QEFLDVMMGE NSSDFQYFQQ LIIKGFLASR KHVDQIVLTI ESMTSASKLP CFSLFTNKEY FIQDIRDRFF LHLTEEQCVV KVTELIQSSI NNWRSIQYDA FQRITNGIL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

GSLLRLFQSD VFDAHLHLYY IYHHKEFGVH EYLVNLLYER SDEILWYLPQ LCEISLNRFE KSSLWKYFLD KSSENMNFSL ILSWMYQAMN EDNIPLISEY AQNMLQEIEM AMVNSKLKPD IRYIEKRLYF WRRSNLSVFQ SLCNQNMKLN DIEDEFKDND NTMLMNRENT NTQLSLAIYS TLPSSETINL NESDDLNQDD NNRIISSDIN TNTIPNNNSK TIKLSSCDVL PDIWIYRIIN DDFSCENKAK IEYIQTKLGL INRQFKNLYS NATCAAYYNP NPGTSLNYKS GVSSSAFGSL NSGFLPTSII SGSSSFGTFS SGGNSNKVSI PYFFFSKLGS PFDLIDVNKI INDIQFRSKS RINLINKSHN FNVQDNSSED TVSIPKTPRD VIRITHPFSP CSETESETNN LDLDDNGTFE EIKLAPDSNV CSDNDNKKIP ILTPNSTKII ECLENLSNEN SGSPIQLNDD ILRNEEIFKV YISEIEKESE TLYYLLKQHR YNYFNRVNQL VSQFLEISNY LQSNINKEHR CEILEFIIEE LNLWLFYSRF SISISRTTFL LNSISLPMPS FNNEFCSRFS ENDSFNSNNK DGNSKGIIGF SHSDYIMDKG LCSLNNCQFL RIIPNECKIY NSRKRVHFLL AIEIADLDDQ DYELGKNSQD LISKDILNYL KNHGIKLKTD NNDENQNRSQ DILLNFLNIL SVGISESIDM GLSQEDFNQI TFIDNKDSDE IEDSSIHDNE LSDEDIFINK DWPQETWSSK IKRYRKESPY SKLKSWGIKT LLLKSSDDLR QEYLASQLLQ QFDWIFKINK LPLWLHPYEI LVIGSHGGFI EYIQDTFSID SLKRRFQTDN LLNCFDQLFK DEELKSKARK CFVESHAAYS LVSYFLQVKD RHNGNFLIDK HGHIIQIDYG FMLSNSPGNV NFEQSPFKLT QEFLDVMMGE NSSDFQYFQQ LIIKGFLASR KHVDQIVLTI ESMTSASKLP CFSLFTNKEY FIQDIRDRFF LHLTEEQCVV KVTELIQSSI NNWRSIQYDA FQRITNGIL  

PCR Forward Primer

TACTTCCAATCCAATGCGGGCAGCCTGCTGCGCCTG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGAATGCCGTTCGTGAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 30, 2015