Clone

Target

IDP95480  

Clone Suffix

8

Sequence Comments

 

Experiment Start Date

Sep 23, 2015  

Experiment End Date

Sep 23, 2015  

Person

Brian Bishop  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

68  

Sequence End

1114  

Reference Sequence Used

GGEFDKSKGS LLRLFQSDVF DAHLHLYYIY HHKEFGVHEY LVNLLYERSD EILWYLPQLC EISLNRFEKS SLWKYFLDKS SENMNFSLIL SWMYQAMNED NIPLISEYAQ NMLQEIEMAM VNSKLKPDIR YIEKRLYFWR RSNLSVFQSL CNQNMKLNDI EDEFKDNDNT MLMNRENTNT QLSLAIYSTL PSSETINLNE SDDLNQDDNN RIISSDINTN TIPNNNSKTI KLSSCDVLPD IWIYRIINDD FSCENKAKIE YIQTKLGLIN RQFKNLYSNA TCAAYYNPNP GTSLNYKSGV SSSAFGSLNS GFLPTSIISG SSSFGTFSSG GNSNKVSIPY FFFSKLGSPF DLIDVNKIIN DIQFRSKSRI NLINKSHNFN VQDNSSEDTV SIPKTPRDVI RITHPFSPCS ETESETNNLD LDDNGTFEEI KLAPDSNVCS DNDNKKIPIL TPNSTKIIEC LENLSNENSG SPIQLNDDIL RNEEIFKVYI SEIEKESETL YYLLKQHRYN YFNRVNQLVS QFLEISNYLQ SNINKEHRCE ILEFIIEELN LWLFYSRFSI SISRTTFLLN SISLPMPSFN NEFCSRFSEN DSFNSNNKDG NSKGIIGFSH SDYIMDKGLC SLNNCQFLRI IPNECKIYNS RKRVHFLLAI EIADLDDQDY ELGKNSQDLI SKDILNYLKN HGIKLKTDNN DENQNRSQDI LLNFLNILSV GISESIDMGL SQEDFNQITF IDNKDSDEIE DSSIHDNELS DEDIFINKDW PQETWSSKIK RYRKESPYSK LKSWGIKTLL LKSSDDLRQE YLASQLLQQF DWIFKINKLP LWLHPYEILV IGSHGGFIEY IQDTFSIDSL KRRFQTDNLL NCFDQLFKDE ELKSKARKCF VESHAAYSLV SYFLQVKDRH NGNFLIDKHG HIIQIDYGFM LSNSPGNVNF EQSPFKLTQE FLDVMMGENS SDFQYFQQLI IKGFLASRKH VDQIVLTIES MTSASKLPCF SLFTNKEYFI QDIRDRFFLH LTEEQCVVKV TELIQSSINN WRSIQYDAFQ RITNGIL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

GGEFDKSKGS LLRLFQSDVF DAHLHLYYIY HHKEFGVHEY LVNLLYERSD EILWYLPQLC EISLNRFEKS SLWKYFLDKS SENMNFSLIL SWMYQAMNED NIPLISEYAQ NMLQEIEMAM VNSKLKPDIR YIEKRLYFWR RSNLSVFQSL CNQNMKLNDI EDEFKDNDNT MLMNRENTNT QLSLAIYSTL PSSETINLNE SDDLNQDDNN RIISSDINTN TIPNNNSKTI KLSSCDVLPD IWIYRIINDD FSCENKAKIE YIQTKLGLIN RQFKNLYSNA TCAAYYNPNP GTSLNYKSGV SSSAFGSLNS GFLPTSIISG SSSFGTFSSG GNSNKVSIPY FFFSKLGSPF DLIDVNKIIN DIQFRSKSRI NLINKSHNFN VQDNSSEDTV SIPKTPRDVI RITHPFSPCS ETESETNNLD LDDNGTFEEI KLAPDSNVCS DNDNKKIPIL TPNSTKIIEC LENLSNENSG SPIQLNDDIL RNEEIFKVYI SEIEKESETL YYLLKQHRYN YFNRVNQLVS QFLEISNYLQ SNINKEHRCE ILEFIIEELN LWLFYSRFSI SISRTTFLLN SISLPMPSFN NEFCSRFSEN DSFNSNNKDG NSKGIIGFSH SDYIMDKGLC SLNNCQFLRI IPNECKIYNS RKRVHFLLAI EIADLDDQDY ELGKNSQDLI SKDILNYLKN HGIKLKTDNN DENQNRSQDI LLNFLNILSV GISESIDMGL SQEDFNQITF IDNKDSDEIE DSSIHDNELS DEDIFINKDW PQETWSSKIK RYRKESPYSK LKSWGIKTLL LKSSDDLRQE YLASQLLQQF DWIFKINKLP LWLHPYEILV IGSHGGFIEY IQDTFSIDSL KRRFQTDNLL NCFDQLFKDE ELKSKARKCF VESHAAYSLV SYFLQVKDRH NGNFLIDKHG HIIQIDYGFM LSNSPGNVNF EQSPFKLTQE FLDVMMGENS SDFQYFQQLI IKGFLASRKH VDQIVLTIES MTSASKLPCF SLFTNKEYFI QDIRDRFFLH LTEEQCVVKV TELIQSSINN WRSIQYDAFQ RITNGIL  

PCR Forward Primer

TACTTCCAATCCAATGCGGGCGGTGAATTTGATAAATCG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGAATGCCGTTCGTGAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 30, 2015