Clone

Target
IDP95362  
Clone Suffix
103
Sequence Comments
IDP95362.1-267.F..pMCSG81  
Experiment Start Date
Feb 01, 2013  
Experiment End Date
Feb 02, 2013  
Person
Robert Jedrzejczak  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG81  
Sequence Start
1  
Sequence End
267  
Reference Sequence Used
MTIKHAEELT EEVILNMNYP DFVALMQQDN TPPGAEYTID YWIKHGCINK KSHLLDLACS TGFSSRECFK KEGASAEGID ISESAVMVAN EKAKKLKANN LLKYYVADAC DLPFEDNTFT HVLGGCNFAF IQNRLIALNE THRCLNHMGS MCISNFYYRR KISDKLINDV YNAINFRPNP LWTLEYWHQF FSERFTLVSE ENHEMESQSE DELKADIYDY IFNRNEFTKG LNGSLQNVFF ERFLKIRRPL NIQRDYQGVT LQIWRKK  
PCR Forward Primer
TACTTCCAATCCAATGCCATGACGATTAAACATGCTGAAGAATTGACG  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTTTGCGCCATATTTGCAATGTAACTCC  
Shipped To Lab
ANL  
Shipped Date
Feb 02, 2013  
Inserted
Dec 19, 2015  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2013-02-03 2013-02-03 Robert Jedrzejczak Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG assay
2015-02-02 2015-02-04 Boguslaw Nocek Argonne National Laboratory standard expression_ANL failed E. coli LB 1 mM IPTG production
2015-03-09 2015-03-09 Boguslaw Nocek Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 1 mM IPTG production