Clone

Target
IDP95709  
Clone Suffix
1
Experiment Start Date
Mar 20, 2016  
Experiment End Date
Mar 20, 2016  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
306  
Sequence End
487  
Reference Sequence Used
RQQRAAQYGR ALQAMEANKY DEARKTLQPL LAAEPGNAWY LDLATDIDLG QNKANEAINR LKNARDLRTN PVLQLNLANA YLQGGQPQEA ANILNRYTFN NKDDSNGWDL LAQAEAALNN RDQELAARAE GYALAGRLDQ AISLLSSASS QVKLGSLQQA RYDARIDQLR QLQERFKPYT KM  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
RQQRAAQYGR ALQAMEANKY DEARKTLQPL LAAEPGNAWY LDLATDIDLG QNKANEAINR LKNARDLRTN PVLQLNLANA YLQGGQPQEA ANILNRYTFN NKDDSNGWDL LAQAEAALNN RDQELAARAE GYALAGRLDQ AISLLSSASS QVKLGSLQQA RYDARIDQLR QLQERFKPYT KM  
PCR Forward Primer
TACTTCCAATCCAATGCGCGTCAGCAACGAGCGGCG  
PCR Backward Primer
TTATCCACTTCCAATGTTACATCTTGGTATAAGGCTTAA  
Shipped To Lab
UT  
Shipped Date
Mar 29, 2016  
Inserted
May 05, 2016  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-04-06 2016-04-06 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2016-05-30 Joanna Sandoval University of Calgary Expression_protocol_Toronto success E. coli LB none production
2016-05-05 Joanna Sandoval University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2016-11-07 Elena Evdokimova University of Calgary Expression_protocol_Toronto success E. coli Se-Met none production