Clone

Target

IDP95709  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Mar 20, 2016  

Experiment End Date

Mar 20, 2016  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

28  

Sequence End

305  

Reference Sequence Used

DSADTLPDMG TSAGSTLSIG QEMQMGDYYV RQLRGSAPLI NDPLLTQYIN SLGMRLVSHA NSVKTPFHFF LINNDEINAF AFFGGNVVLH SALFRYSDNE SQLASVMAHE ISHVTQRHLA RAMEDQQRNA PLTWVGALGS ILLAMASPQA GMAALTGTLA GTRQGMISFT QQNEQEADRI GIQVLQRSGF DPQAMPTFLE KLLDQARYSS RPPEILLTHP LPESRLADAR NRANQMRPMV VQSSEDFYLA KARTLGMYNS GRNQLTSDLL DEWAKGNV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

DSADTLPDMG TSAGSTLSIG QEMQMGDYYV RQLRGSAPLI NDPLLTQYIN SLGMRLVSHA NSVKTPFHFF LINNDEINAF AFFGGNVVLH SALFRYSDNE SQLASVMAHE ISHVTQRHLA RAMEDQQRNA PLTWVGALGS ILLAMASPQA GMAALTGTLA GTRQGMISFT QQNEQEADRI GIQVLQRSGF DPQAMPTFLE KLLDQARYSS RPPEILLTHP LPESRLADAR NRANQMRPMV VQSSEDFYLA KARTLGMYNS GRNQLTSDLL DEWAKGNV  

PCR Forward Primer

TACTTCCAATCCAATGCGGACAGCGCAGACACCTTGC  

PCR Backward Primer

TTATCCACTTCCAATGTTAAACGTTTCCTTTCGCCCA  

Shipped To Lab

UT  

Shipped Date

Mar 29, 2016  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2016