Clone

Target

IDP95711  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Mar 20, 2016  

Experiment End Date

Mar 20, 2016  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

28  

Sequence End

257  

Reference Sequence Used

SQQARHSAVQ KTHLAKTTER KKKTTTKNEK KKTTAQTKKT ASSKSKSTSS RTALASKRKA SKTAANLVTE KCTTRKGHKA KCTKVTKIAD AHKVRVQKAQ KTAMNKLMGQ IGKPYRWGGT SPRTGFDCSG LVYYAYKDLV KFRIPRTANE MYHLRDAAPV NRGELQNGDL VFFRTQGRGT ADHVGVYVGN GKFIQSPRSG QDIQITSLSE DYWVRHYVGA RRVMTPKTIR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGTCTCAACAGGCGCGGCAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCGGATGGTTTTCGGCG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2016