Clone

Target
IDP95712  
Clone Suffix
1
Sequence Comments
C244Y  
Experiment Start Date
Mar 20, 2016  
Experiment End Date
Mar 20, 2016  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
262  
Reference Sequence Used
MDAIKGSELQ IPDAIFAWVL DGQGGVKPLA DNDIIDKEKP CWLHLNYTHS DSADWLAATP LLPNNVRDAL AGESTRPRVT RIGDGALITL RCINGSTDER PDQLVAMRLY MDERLIVSTR QRKVLALDDV LGDLKEGNGP TDCGSWLVEV CDALTDHASE FIEQLHDRII DLEDDLLDQQ VPPRGFLALL RKQLIVMRRY MAPQRDVYAR LASERLPWMS DDQRRRMQDI AERLGRGLDE IDSCIARTAI MSDEIAQIMQ ES  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MDAIKGSELQ IPDAIFAWVL DGQGGVKPLA DNDIIDKEKP CWLHLNYTHS DSADWLAATP LLPNNVRDAL AGESTRPRVT RIGDGALITL RCINGSTDER PDQLVAMRLY MDERLIVSTR QRKVLALDDV LGDLKEGNGP TDCGSWLVEV CDALTDHASE FIEQLHDRII DLEDDLLDQQ VPPRGFLALL RKQLIVMRRY MAPQRDVYAR LASERLPWMS DDQRRRMQDI AERLGRGLDE IDSYIARTAI MSDEIAQIMQ ES  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGACGCGATTAAAGGCTC  
PCR Backward Primer
TTATCCACTTCCAATGTTACGACTCCTGCATAATCTG  
Shipped To Lab
UT  
Shipped Date
Mar 29, 2016  
Inserted
May 05, 2016  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-04-06 2016-04-06 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2016-07-13 Joanna Sandoval University of Calgary Expression_protocol_Toronto success E. coli LB none production
2016-05-05 Joanna Sandoval University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production