Clone

Target

IDP95712  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Mar 20, 2016  

Experiment End Date

Mar 20, 2016  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

35  

Sequence End

262  

Reference Sequence Used

IDKEKPCWLH LNYTHSDSAD WLAATPLLPN NVRDALAGES TRPRVTRIGD GALITLRCIN GSTDERPDQL VAMRLYMDER LIVSTRQRKV LALDDVLGDL KEGNGPTDCG SWLVEVCDAL TDHASEFIEQ LHDRIIDLED DLLDQQVPPR GFLALLRKQL IVMRRYMAPQ RDVYARLASE RLPWMSDDQR RRMQDIAERL GRGLDEIDSC IARTAIMSDE IAQIMQES  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

IDKEKPCWLH LNYTHSDSAD WLAATPLLPN NVRDALAGES TRPRVTRIGD GALITLRCIN GSTDERPDQL VAMRLYMDER LIVSTRQRKV LALDDVLGDL KEGNGPTDCG SWLVEVCDAL TDHASEFIEQ LHDRIIDLED DLLDQQVPPR GFLALLRKQL IVMRRYMAPQ RDVYARLASE RLPWMSDDQR RRMQDIAERL GRGLDEIDSC IARTAIMSDE IAQIMQES  

PCR Forward Primer

TACTTCCAATCCAATGCGATCGACAAAGAGAAGCCCTG  

PCR Backward Primer

TTATCCACTTCCAATGTTACGACTCCTGCATAATCTG  

Shipped To Lab

UT  

Shipped Date

Mar 29, 2016  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2016