Clone

Target

IDP95713  

Clone Suffix

1

Sequence Comments

E346G  

Experiment Start Date

Mar 20, 2016  

Experiment End Date

Mar 20, 2016  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

85  

Sequence End

483  

Reference Sequence Used

TPRTDWHRYL EQNVFKQVHP LVTEIELRAK QNLPTDLDMY TGELPLDLIP SFDFPALNWK QFLKEEAQDE LGYLPLRKEI QAITATEYQL HLPSESLLLT SGAQQALFLV LQVLLRQGDS VAVEDPSFFY ALPIFQAAGV RLFGVPMTEE GIDLEALEQT IRQHRIKMVM VNPSFQNPTG TVMPLRKREQ LVKVCQTYQV PILEDDVFGQ LSFIPKTEIP PLKKLDPDNV LYIGSLSKIL GSTTKIGWLS APASVTKQIA EARKMMDVSL SIFPQMLAKM AIEDPSFSEK ITLLNKQVEQ RATAVYQVFK SLSEWEVSPV KGGFYLWAHW RQGALKPEDW QVFLREGVLV APSVAFSEKR GSIRLNCSRI SPEEMPLFCE RMVRITRQLS ENRQTKIEQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

TPRTDWHRYL EQNVFKQVHP LVTEIELRAK QNLPTDLDMY TGELPLDLIP SFDFPALNWK QFLKEEAQDE LGYLPLRKEI QAITATEYQL HLPSESLLLT SGAQQALFLV LQVLLRQGDS VAVEDPSFFY ALPIFQAAGV RLFGVPMTEE GIDLEALEQT IRQHRIKMVM VNPSFQNPTG TVMPLRKREQ LVKVCQTYQV PILEDDVFGQ LSFIPKTEIP PLKKLDPDNV LYIGSLSKIL GSTTKIGWLS APASVTKQIA EARKMMDVSL SIFPQMLAKM AIEDPSFSEK ITLLNKQVEQ RATAVYQVFK SLSEWEVSPV KGGFYLWAHW RQGALKPEDW QVFLRGGVLV APSVAFSEKR GSIRLNCSRI SPEEMPLFCE RMVRITRQLS ENRQTKIEQ  

PCR Forward Primer

TACTTCCAATCCAATGCGACGCCACGAACGGATTGGC  

PCR Backward Primer

TTATCCACTTCCAATGTTACTGTTCTATTTTTGTTTGTCT  

Shipped To Lab

UT  

Shipped Date

May 02, 2016  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2016