Clone

Target

IDP95713  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Mar 20, 2016  

Experiment End Date

Mar 20, 2016  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

483  

Reference Sequence Used

MWQLKKEQNV PIYRSIMEMI VQKIQTGELL PGEKIPSERK LAEYLSVNRS TVVHALDELV DLGWIVRKRG SGTIVNEGKW GVMMTPRTDW HRYLEQNVFK QVHPLVTEIE LRAKQNLPTD LDMYTGELPL DLIPSFDFPA LNWKQFLKEE AQDELGYLPL RKEIQAITAT EYQLHLPSES LLLTSGAQQA LFLVLQVLLR QGDSVAVEDP SFFYALPIFQ AAGVRLFGVP MTEEGIDLEA LEQTIRQHRI KMVMVNPSFQ NPTGTVMPLR KREQLVKVCQ TYQVPILEDD VFGQLSFIPK TEIPPLKKLD PDNVLYIGSL SKILGSTTKI GWLSAPASVT KQIAEARKMM DVSLSIFPQM LAKMAIEDPS FSEKITLLNK QVEQRATAVY QVFKSLSEWE VSPVKGGFYL WAHWRQGALK PEDWQVFLRE GVLVAPSVAF SEKRGSIRLN CSRISPEEMP LFCERMVRIT RQLSENRQTK IEQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTGGCAACTTAAAAAAGAACA  

PCR Backward Primer

TTATCCACTTCCAATGTTACTGTTCTATTTTTGTTTGTCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2016