Clone

Target
IDP95716  
Clone Suffix
1
Experiment Start Date
Mar 20, 2016  
Experiment End Date
Mar 20, 2016  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
23  
Sequence End
184  
Reference Sequence Used
PKKTTLRDKA MVNYAFDYLS SPGSLPFTTA ATELSAIHGH STSQYRLGEF YLHGSDGKPL DYTQARYWYE QSAEQENPRA QSKLGWIYLK GLGVKPDTRK AILWYKEAAE QGYAHAQYTL GLIYRNGSGI NVNHYESQKW LKLAAKQHYK NAERLLAGLP AH  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
PKKTTLRDKA MVNYAFDYLS SPGSLPFTTA ATELSAIHGH STSQYRLGEF YLHGSDGKPL DYTQARYWYE QSAEQENPRA QSKLGWIYLK GLGVKPDTRK AILWYKEAAE QGYAHAQYTL GLIYRNGSGI NVNHYESQKW LKLAAKQHYK NAERLLAGLP AH  
PCR Forward Primer
TACTTCCAATCCAATGCGCCCAAGAAAACGACGTTGCG  
PCR Backward Primer
TTATCCACTTCCAATGTTAATGTGCGGGAAGCCCG  
Shipped To Lab
UT  
Shipped Date
Mar 29, 2016  
Inserted
May 05, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-04-06 2016-04-06 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2016-04-11 2016-04-11 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay