Clone

Target
IDP95729  
Clone Suffix
7
Experiment Start Date
Apr 09, 2016  
Experiment End Date
Apr 09, 2016  
Person
Brian Bishop  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
252  
Reference Sequence Used
IVSYVVSGKS VDMYIERAGD ITWEKDAEVT GNSPRLDVAL DESGDFSLVE DDGPPMRGGG GSGGGGSGAL WDVPAPKEVK KGETTDGVYR VMTRRLLGST QVGVGVMQEG VFHTMWHVTK GSALRSGEGR LDPYWGDVKQ DLVSYCGPWK LDAAWDGHSE VQLLAVPPGE RARNIQTLPG IFKTKDGDIG AVALDYPAGT SGSPILDKCG RVIGLYGNGV VIKNGSYVSA ITQGRREEET PVECFEPSML KK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATCGTTTCTTATGTGGTTAGCG  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTTTCAGCATCGATGGTT  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-04-11 2016-04-15 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2016-04-15 2016-04-15 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay