Clone

Target
IDP95732  
Clone Suffix
6
Experiment Start Date
May 08, 2016  
Experiment End Date
May 08, 2016  
Person
Brian Bishop  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pCPD  
Sequence Start
1  
Sequence End
242  
Reference Sequence Used
MGETLGEKWK ARLNQMSALE FYSYKKSGIT EVCREEARRA LKDGVATGGH AVSRGSAKLR WLVERGYLQP YGKVIDLGCG RGGWSYYAAT IRKVQEVKGY TKGGPGHEEP VLVQSYGWNI VRLKSGVDVF HMAAEPCDTL LCDIGESSSS PEVEEARTLR VLSMVGDWLE KRPGAFCIKV LCPYTSTMME TLERLQRRYG GGLVRVPLSR NSTHEMYWVS GAKSNTIKSV STTSQLLLGR MDG  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MGETLGEKWK ARLNQMSALE FYSYKKSGIT EVCREEARRA LKDGVATGGH AVSRGSAKLR WLVERGYLQP YGKVIDLGCG RGGWSYYAAT IRKVQEVKGY TKGGPGHEEP VLVQSYGWNI VRLKSGVDVF HMAAEPCDTL LCDIGESSSS PEVEEARTLR VLSMVGDWLE KRPGAFCIKV LCPYTSTMME TLERLQRRYG GGLVRVPLSR NSTHEMYWVS GAKSNTIKSV STTSQLLLGR MDG  
PCR Forward Primer
GTCTCTCCCATGGGAGAGACCCTGGGC  
PCR Backward Primer
TGCTGCCCCGCCGTCCATGCGTCCTAAT  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-26 2016-05-26 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2016-05-26 2016-05-26 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2016-05-26 2016-05-26 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay