Clone

Target
IDP95842  
Clone Suffix
1
Experiment Start Date
Feb 16, 2017  
Experiment End Date
Feb 16, 2017  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
162  
Reference Sequence Used
MIQRVYVALG SNLAEPREQI QAALDAFERL PETRLVAVSP LYISDPLGPA DQPRFVNGVA ALDTNLAPLD LLDALQAIEL EQGRVRDLRW GPRTLDLDIL LFGEQLLDLP RLKVPHYHMQ ARAFVLYPLA DLAPDLRLPD GRHLPELLAA CPFEGIERLP GA  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGATCCAGCGCGTCTACG  
PCR Backward Primer
TTATCCACTTCCAATGTCAGGCGCCCGGAAGGCG  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-02-16 2017-02-16 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2017-03-07 2017-03-07 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay