Clone

Target

IDP95847  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Feb 16, 2017  

Experiment End Date

Feb 16, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

252  

Reference Sequence Used

MDKRLWVGVV SIFPEMFRAI SDYGITSRAV KQGLLTLTCW NPRDYTEDRH QTVDDRPFGG GPGMVMKIKP LEGALADARQ AAGGRKAKVI YLSPQGRQLT QAGVRELAEE EALILIAGRY EGIDERFIEE HVDEEWSIGD YVLSGGELPA MVLVDAVTRL LPGALGHADS AEEDSFTDGL LDCPHYTRPE VYADKRVPEV LLSGNHEHIR RWRLQQALGR TWERRADLLD SRSLSGEEQK LLAEYIRQRD DS  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MDKRLWVGVV SIFPEMFRAI SDYGITSRAV KQGLLTLTCW NPRDYTEDRH QTVDDRPFGG GPGMVMKIKP LEGALADARQ AAGGRKAKVI YLSPQGRQLT QAGVRELAEE EALILIAGRY EGIDERFIEE HVDEEWSIGD YVLSGGELPA MVLVDAVTRL LPGALGHADS AEEDSFTDGL LDCPHYTRPE VYADKRVPEV LLSGNHEHIR RWRLQQALGR TWERRADLLD SRSLSGEEQK LLAEYIRQRD DS  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGACAAGCGTTTGTGGGT  

PCR Backward Primer

TTATCCACTTCCAATGTTAACTATCGTCCCGCTGAC  

Shipped To Lab

NU  

Shipped Date

Mar 20, 2017  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 27, 2017