Clone

Target

IDP95857  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Feb 16, 2017  

Experiment End Date

Feb 16, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

233  

Reference Sequence Used

MNKILLQCDN LCKRYQEGSV QTDVLHNVSF SVGEGEMMAI VGSSGSGKST LLHLLGGLDT PTSGDVIFNG QPMSKLSSAA KAELRNQKLG FIYQFHHLLP DFTALENVAM PLLIGKKKPA EINSRALEML KAVGLDHRAN HRPSELSGGE RQRVAIARAL VNNPRLVLAD EPTGNLDARN ADSIFQLLGE LNRLQGTAFL VVTHDLQLAK RMSRQLEMRD GRLTAELSLM GAE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNKILLQCDN LCKRYQEGSV QTDVLHNVSF SVGEGEMMAI VGSSGSGKST LLHLLGGLDT PTSGDVIFNG QPMSKLSSAA KAELRNQKLG FIYQFHHLLP DFTALENVAM PLLIGKKKPA EINSRALEML KAVGLDHRAN HRPSELSGGE RQRVAIARAL VNNPRLVLAD EPTGNLDARN ADSIFQLLGE LNRLQGTAFL VVTHDLQLAK RMSRQLEMRD GRLTAELSLM GAE  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATAAGATCCTGTTGCAATG  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCCGCCCCCATCAGG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 27, 2017