Clone

Target

IDP95863  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Feb 16, 2017  

Experiment End Date

Feb 16, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

262  

Reference Sequence Used

MTRIHPTAIV EPGAQIDESV EIGPYAIVGP HVTIGARTTI GSHSVIEGHT TLGEDNRIGH YASVGGRPQD MKYKDEPTKL VIGNRNTIRE FTTIHTGTVQ DVGVTTLGDD NWIMAYVHIG HDCRVGNNVI LSSNAQMAGH VEIGDFAIIG GMSGVHQFVR IGAHSMLGGA SALVQDIPPF VIAAGNKAEP HGINVEGLRR RGFSADAISA LRSAYRVLYK NGLSLEEAKV QLRELAGAGG DGDAPVKALV EFIDASQRGI IR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTRIHPTAIV EPGAQIDESV EIGPYAIVGP HVTIGARTTI GSHSVIEGHT TLGEDNRIGH YASVGGRPQD MKYKDEPTKL VIGNRNTIRE FTTIHTGTVQ DVGVTTLGDD NWIMAYVHIG HDCRVGNNVI LSSNAQMAGH VEIGDFAIIG GMSGVHQFVR IGAHSMLGGA SALVQDIPPF VIAAGNKAEP HGINVEGLRR RGFSADAISA LRSAYRVLYK NGLSLEEAKV QLRELAGAGG DGDAPVKALV EFIDASQRGI IR  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACCAGGATTCATCCTACC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCGGATGATGCCGCGT  

Shipped To Lab

ANL  

Shipped Date

Mar 20, 2017  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 27, 2017