Clone

Target

IDP95873  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Feb 16, 2017  

Experiment End Date

Feb 16, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

512  

Reference Sequence Used

MNLLKSLAAV SSITMLSRVL GFVRDTILAR IFGAGLATDA FFVAFKLPNL LRRIFAEGAF SQAFVPILAE YKNQQGEEAT RTFIAYVSGL LTLVLALVTA LGILAAPWVI WVTAPGFADT PEKFALTTDL LRVTFPYILL ISLSSLAGAI LNTWNRFSVP AFVPTLLNVA MIGFALFLTP YFDPPVMVLG WAVLAGGLLQ LLYQLPHLRK IGMLVLPRLN LRDSGVWRVM KLMLPAILGV SVSQISLIIN TIFASFLAAG SVSWMYYADR LMELPSGVLG VALGTILLPM LAKTYSNKDR HEYSRLLDWG LRLCFLLVLP CSLALAILAE PLTVSLFQYG KFTTVDAAMT QRALVAYSVG LLGIILVKVL APGFYAQQNI RTPVKIALFT LVSTQLMNLA FIGPLQHAGL ALSIGLAACL NAGLLYWQLR KQRLYLPQPG WAKFLVKLVV AVLAMSAVLL ATMHWLPAWE QGAMLERFLR LGLLVVAGLL AYFGMLALLG FRLRDFSRRA VL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATCTTCTCAAATCATTGGC  

PCR Backward Primer

TTATCCACTTCCAATGTCACAGCACCGCCCGGCG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 27, 2017