Clone

Target
IDP96117  
Clone Suffix
1
Experiment Start Date
May 05, 2017  
Experiment End Date
May 05, 2017  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
203  
Reference Sequence Used
MRLWLIRHGE TQANIDGLYS GHAPTPLTAR GIEQAQNLHT LLHGVSFDLV LCSELERAQH TARLVLSDRQ LPVQIIPELN EMFFGDWEMR HHRDLMQEDA ENYSAWCNDW QHAIPTNGEG FQAFSQRVER FIARLSEFQH YQNILVVSHQ GVLSLLIARL IGMPAEAMWH FRVDQGCWSA IDINQKFATL RVLNSRAIGV ENA  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MRLWLIRHGE TQANIDGLYS GHAPTPLTAR GIEQAQNLHT LLHGVSFDLV LCSELERAQH TARLVLSDRQ LPVQIIPELN EMFFGDWEMR HHRDLMQEDA ENYSAWCNDW QHAIPTNGEG FQAFSQRVER FIARLSEFQH YQNILVVSHQ GVLSLLIARL IGMPAEAMWH FRVDQGCWSA IDINQKFATL RVLNSRAIGV ENA  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCGACTGTGGTTAATTCGT  
PCR Backward Primer
TTATCCACTTCCAATGTCATGCATTCTCGACCCCG  
Shipped To Lab
ANL  
Shipped Date
Jul 05, 2017  
Inserted
Jun 17, 2017  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-05-05 2017-05-05 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2018-04-11 2018-04-13 Natalia Maltseva Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 0.5 mM IPTG production