Clone

Target
IDP96122  
Clone Suffix
1
Experiment Start Date
May 05, 2017  
Experiment End Date
May 05, 2017  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
322  
Reference Sequence Used
MLNSFKLSLQ YILPKLWLTR LAGWGASKRA GWLTKLVIDL FVKYYKVDMK EAQKPDTASY RTFNEFFVRP LRDEVRPIDT DPNVLVMPAD GVISQLGKIE EDKILQAKGH NYSLEALLAG NYLMADLFRN GTFVTTYLSP RDYHRVHMPC NGILREMIYV PGDLFSVNHL TAQNVPNLFA RNERVICLFD TEFGPMAQIL VGATIVGSIE TVWAGTITPP REGIIKRWTW PAGENDGSVA LLKGQEMGRF KLGSTVINLF APGKVNLVEQ LESLSVTKIG QPLAVSTETF VTPDAEPAPL PAEEIEAEHD ASPLVDDKKD QV  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MLNSFKLSLQ YILPKLWLTR LAGWGASKRA GWLTKLVIDL FVKYYKVDMK EAQKPDTASY RTFNEFFVRP LRDEVRPIDT DPNVLVMPAD GVISQLGKIE EDKILQAKGH NYSLEALLAG NYLMADLFRN GTFVTTYLSP RDYHRVHMPC NGILREMIYV PGDLFSVNHL TAQNVPNLFA RNERVICLFD TEFGPMAQIL VGATIVGSIE TVWAGTITPP REGIIKRWTW PAGENDGSVA LLKGQEMGRF KLGSTVINLF APGKVNLVEQ LESLSVTKIG QPLAVSTETF VTPDAEPAPL PAEEIEAEHD ASPLVDDKKD QV  
PCR Forward Primer
TACTTCCAATCCAATGCGATGTTAAATTCATTTAAACTTTCGC  
PCR Backward Primer
TTATCCACTTCCAATGTTAGACCTGGTCTTTTTTGTCG  
Shipped To Lab
ANL  
Shipped Date
Jul 05, 2017  
Inserted
Jun 17, 2017  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-05-05 2017-05-05 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2017-09-25 2017-09-27 Rory Mulligan Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production
2018-04-11 2018-04-13 Natalia Maltseva Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 0.5 mM IPTG production