Clone

Target
IDP96123  
Clone Suffix
1
Experiment Start Date
May 05, 2017  
Experiment End Date
May 05, 2017  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
279  
Reference Sequence Used
MKNNPLISIY MPTWNRQQLA IRAIKSVLRQ DYSNWEMIIV DDCSTSWEQL QQYVTALNDP RITYIHNDIN SGACAVRNQA IMLAQGEYIT GIDDDDEWTP NRLSVFLAHK QQLVTHAFLY ANDYVCQGEV YSQPASLPLY PKSPYSRRLF YKRNIIGNQV FTWAWRFKEC LFDTELKAAQ DYDIFLRMVV EYGEPWKVEE ATQILHINHG EMQITSSPKK FSGYFHFYRK HKDKFDRASK KYQLFTLYQI RNKRMTWRTL LTLLSVRNGK RLADGIRGR  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAAACAATCCGCTGATCT  
PCR Backward Primer
TTATCCACTTCCAATGTTAGCGCCCCCGAATACCA  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-05-05 2017-05-05 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay