Clone

Target

IDP96124  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

May 05, 2017  

Experiment End Date

May 05, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

506  

Reference Sequence Used

MVSSTTPSSG EYLLEMSGIN KSFPGVKALD NVNLKVRPHS IHALMGENGA GKSTLLKCLF GIYQKDSGTI LFQGKEIDFH SAKEALENGI SMVHQELNLV LQRSVMDNMW LGRYPTKGMF VDQDKMYRET KAIFDELDID IDPRARVGTL SVSQMQMIEI AKAFSYNAKI VIMDEPTSSL TEKEVNHLFT IIRKLKERGC GIVYISHKME EIFQLCDEVT VLRDGQWIAT EPLAGLTMDK IIAMMVGRSL NQRFPDKENK PGEVILEVRN LTSLRQPSIR DVSFDLHKGE ILGIAGLVGA KRTDIVETLF GIREKSAGTI TLHGKQINNH NANEAINHGF ALVTEERRST GIYAYLDIGF NSLISNIRNY KNKVGLLDNS RMKSDTQWVI DSMRVKTPGH RTQIGSLSGG NQQKVIIGRW LLTQPEILML DEPTRGIDVG AKFEIYQLIA ELAKKGKGII IISSEMPELL GITDRILVMS NGLVSGIVDT KTTTQNEILR LASLHL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MVSSTTPSSG EYLLEMSGIN KSFPGVKALD NVNLKVRPHS IHALMGENGA GKSTLLKCLF GIYQKDSGTI LFQGKEIDFH SAKEALENGI SMVHQELNLV LQRSVMDNMW LGRYPTKGMF VDQDKMYRET KAIFDELDID IDPRARVGTL SVSQMQMIEI AKAFSYNAKI VIMDEPTSSL TEKEVNHLFT IIRKLKERGC GIVYISHKME EIFQLCDEVT VLRDGQWIAT EPLAGLTMDK IIAMMVGRSL NQRFPDKENK PGEVILEVRN LTSLRQPSIR DVSFDLHKGE ILGIAGLVGA KRTDIVETLF GIREKSAGTI TLHGKQINNH NANEAINHGF ALVTEERRST GIYAYLDIGF NSLISNIRNY KNKVGLLDNS RMKSDTQWVI DSMRVKTPGH RTQIGSLSGG NQQKVIIGRW LLTQPEILML DEPTRGIDVG AKFEIYQLIA ELAKKGKGII IISSEMPELL GITDRILVMS NGLVSGIVDT KTTTQNEILR LASLHL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTCAGCTCAACGACTCC  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAGGTGCAAAGACGCAAG  

Shipped To Lab

ANL  

Shipped Date

Jul 05, 2017  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 17, 2017