Clone

Target

IDP96125  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

May 05, 2017  

Experiment End Date

May 05, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

500  

Reference Sequence Used

MTTDQHQEIL RTEGLSKFFP GVKALDNVDF SLRRGEIMAL LGENGAGKST LIKALTGVYH ADRGTIWLEG QAISPKNTAH AQQLGIGTVY QEVNLLPNMS VADNLFIGRE PKRFGLLRRK EMEKRATELM ASYGFSLDVR EPLNRFSVAM QQIVAICRAI DLSAKVLILD EPTASLDTQE VELLFDLMRQ LRDRGVSLIF VTHFLDQVYQ VSDRITVLRN GSFVGCRETC ELPQIELVKM MLGRELDTHA LQRAGRTLLS DKPVAAFKNY GKKGTIAPFD LEVRPGEIVG LAGLLGSGRT ETAEVIFGIK PADSGTALIK GKPQNLRSPH QASVLGIGFC PEDRKTDGII AAASVRENII LALQAQRGWL RPISRKEQQE IAERFIRQLG IRTPSTEQPI EFLSGGNQQK VLLSRWLLTR PQFLILDEPT RGIDVGAHAE IIRLIETLCA DGLALLVISS ELEELVGYAD RVIIMRDRKQ VAEIPLAELS VPAIMNAIAA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACGACCGACCAACACC  

PCR Backward Primer

TTATCCACTTCCAATGTTACGCCGCAATGGCGTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 17, 2017