Clone

Target

IDP96132  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

May 05, 2017  

Experiment End Date

May 05, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

422  

Reference Sequence Used

MYDFVIIGGG IIGMSTAMQL IDVYPDARIA LLEKESAPAC HQTGHNSGVI HAGVYYTPGS LKAQFCLAGN RATKAFCDQN GIRYDNCGKM LVATSDLEME RMRALWERTA ANGIEREWLN ADELREREPN ITGLGGIFVP SSGIVSYRDV TAAMAKIFQS RGGEIIYNAE VSGLNEHKNG VVIRTRQGGE YEASTLISCS GLMADRLVKM LGLEPGFIIC PFRGEYFRLA PEHNQIVNHL IYPIPDPAMP FLGVHLTRMI DGSVTVGPNA VLAFKREGYR KRDFSFSDTL EILGSSGIRR VLQNHLRSGL GEMKNSLCKS GYLRLVQKYC PRLSLSDLQP WPAGVRAQAV SPDGKLIDDF LFVTTPRTIH TCNAPSPAAT SAIPIGAHIV SKVQTLLASQ SNPGRTLRAA RSVDALHAAF NQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTATGATTTTGTGATTATTGGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGATTAAATGCGGCGTGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 17, 2017