Clone

Target

IDP96133  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

May 05, 2017  

Experiment End Date

May 05, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

384  

Reference Sequence Used

MESLMPWDAR DTMSLRTEFV LFASQDGANI RSLCRRFGIS PATGYKWLQR WAQEGAAGLQ DRPRIPHHSP NRSSDDITAL LRMAHDRHER WGARKIKRWL EDQGHTMPAF STVHNLMARH GLLPGASPGI PATGRFEHDA PNRLWQMDFK GHFPFGGGRC HPLTLLDDHS RFSLCLAHCT DERRETVQQQ LVSVFERYGL PDRMTMDNGS PWGDTTGTWT ALELWLMRLG IRVGHSRPYH PQTQGKLERF HRSLKAEVLQ GKWFADSGEL QRAFDHWRTV YNLERPHEAL DMAVPGSRYQ PSARQYSGNT TPPEYDEGVM VRKVDISGKL SVKGVSLSAG KAFRGERVGL KEMQEDGSYE VWWYSTKVGV IDLKKKSITM GKGC  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAGTCGCTTATGCCCTG  

PCR Backward Primer

TTATCCACTTCCAATGTTAACATCCTTTACCCATGGTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 17, 2017