Clone

Target

IDP02031  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

436  

Reference Sequence Used

MLKKYGVATA LSLLIISAPL TGAHADTIND MQKRQNEIEQ KKSEIDKNID SKNSELNHLE SAEKDAAKEL ESLMKSLDDT NKKLKEQEDK VSSENEKLKK LQKEMEKLRN DIRDRQKVLD NRARAIQTTG TATSYLDMIF EADDFKELVD RVTVVSAIVK ADQNIMQDQK DDQDKLKVAE STSEKKLENL KVLAVELEVS KNNMESQKQE KNDLVMALAN KKDLTKSEQT LLASEQGALT DEEKRLASNI AGEKAKQEAA IKAAEEKRMQ EAAAASSAKS AAVVKQPSSS SNEATETVSS GGGQFIKPAS GILTSGFSER TNPVTGKYES HKGQDIAGGG TVTVSAAASG TVVFSGFGAS GSGFGGYGYV VKIDHGNGFQ TLYGHMRAGS LKVVTGQQVS QGQPIGIMGS TGQSTGQHLH FEIHKNGIPV DPAPYI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTAAAAAAATATGGAGTAGCA  

PCR Backward Primer

TTATCCACTTCCAATGTTAAATATATGGTGCTGGATCG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009