Clone

Target
IDP02766  
Clone Suffix
1
Experiment Start Date
Apr 20, 2009  
Experiment End Date
Apr 20, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
169  
Reference Sequence Used
MKIQLQKSFY FELKNAIRCH YNELLTRCGV DTIYGYSILT DDCVNNIGPV ANEERLIKVN KSDPLYNYYR YGAIEWSEWD DFGMFDEVNK IIKKYHNIVE DDFNIRVHTL LKETLTVLME LELEGLFGDR NDNRFIVICV TDSSNEIMIE SARLLNTLKT YEEYASEFA  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAATACAGCTGCAAAAAAG  
PCR Backward Primer
TTATCCACTTCCAATGTTATGCAAATTCTGAAGCATATTC  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2009-04-29 2009-04-30 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2010-05-24 2010-05-25 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay