Clone

Target

IDP00312  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

414  

Reference Sequence Used

MEKRRVVITG LGVVSPLGNK VSDMWQALLA GKSGVKPITR FDASSFPTQI AAEVRDFDPA LVLDLKSIRK TDVFVQFAME SARQAWEDSG LEINETNAPR VGVAIGSGIG GMPWIEKNYD ALLTSGPRKI SPFFIPGAII NMASGMVSIK YDLKGPNISI VTACTTGLHN IGHAARMIAH NDADAMIAGG TEMASTPLGI GGFAAVRALS TRNDEPEKAS RPWDKGRDGF VLGEGAACVV VEELEHAKKR NATIYAEIIG FGMSGDAYHM TRPDPEAEGF TTCMKNSLRD AGIAPERVDY INAHGTSTPA ADPLEARAIK KTFGDHAYKL AVSSTKSMTG HMLGAAGALE TVISVLAIRD NTAPPTINLE NPDEGCDLDF VPNEAREMKI DTVMSNSFGF GGTNGTLVLS RVFD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAGAAACGGCGCGTTG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTCAAAAACTCGGCTTAAAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009