Clone

Target

IDP02507  

Clone Suffix

1

Sequence Comments

90: V -> A  

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

552  

Reference Sequence Used

MIKKFLRLIY IFYVINKYCL LNEPIRATKI KTLRALLLLN PFYYSRRVRR LEHGVRIREA LEKLGPIFIK FGQALSVRAD LLPPDVIKEV SKLQDNVPLF DNKIAAEQIE KAAKKPINEI FKSFESSPLA SASVAQVHAA ILQNDDKVVV KVLRPGIEKI LKLDTSLMLF FATLLSKLKE IRRFKPVEIV KEINQSFFDE LDLVREASNA SQIRRNFEDS AIHYVPKIYW EYTSSTVMVM ERVGGVRVSD IETLDALGVD RRLLAQRGVE IFYSQVFDDC FFHADMHPGN MFIDVSNPAD PKYISIDFGI VGTLNRDDQR YLAGNFLAFF KRDYRKVAEL HIESGWVPSD TRVDVLESAI RTVCEPIFEK PMKEISLGYT LMQLFAVARR FNMNIQPQLT LLQKTLFHVE GLGQKLCPEL NIWETSRPIL EKWMKEQMGL RGFYHRSMEN MPRVSDKLPE LPRMVFDILQ QTQINLKNTT TSTQFNNLKE PKKKYRFALG CGLVLTTIGV IYTLNKDTTP LIKLQNFISN YSTSFIIVGV ACLIYYSFKK EK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MIKKFLRLIY IFYVINKYCL LNEPIRATKI KTLRALLLLN PFYYSRRVRR LEHGVRIREA LEKLGPIFIK FGQALSVRAD LLPPDVIKEA SKLQDNVPLF DNKIAAEQIE KAAKKPINEI FKSFESSPLA SASVAQVHAA ILQNDDKVVV KVLRPGIEKI LKLDTSLMLF FATLLSKLKE IRRFKPVEIV KEINQSFFDE LDLVREASNA SQIRRNFEDS AIHYVPKIYW EYTSSTVMVM ERVGGVRVSD IETLDALGVD RRLLAQRGVE IFYSQVFDDC FFHADMHPGN MFIDVSNPAD PKYISIDFGI VGTLNRDDQR YLAGNFLAFF KRDYRKVAEL HIESGWVPSD TRVDVLESAI RTVCEPIFEK PMKEISLGYT LMQLFAVARR FNMNIQPQLT LLQKTLFHVE GLGQKLCPEL NIWETSRPIL EKWMKEQMGL RGFYHRSMEN MPRVSDKLPE LPRMVFDILQ QTQINLKNTT TSTQFNNLKE PKKKYRFALG CGLVLTTIGV IYTLNKDTTP LIKLQNFISN YSTSFIIVGV ACLIYYSFKK EK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTAAAAAATTTCTAAGGCTTA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTCTCCTTTTTAAAACTATAG  

Shipped To Lab

ANL  

Shipped Date

Jul 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009