Clone

Target

IDP90790  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

200  

Reference Sequence Used

MQKFIIHKGI ACPLEYANID TDQIIPKQFL LAVSKQGFGK HLFHDLRYLD DKESVLNMDF NLNKKEYQNS SILVSFENFG SGSSREHAPW ALVDYGIRAI IAPSFADIFK NNALGNGLLT IELAKDEVLE IVDELKKSQD KNIEISLLEK RVFFKDKIFS FDLDDFHRIC LLEGLDNIAL TLKHEAQIKA YEKNSKSFLV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MQKFIIHKGI ACPLEYANID TDQIIPKQFL LAVSKQGFGK HLFHDLRYLD DKESVLNMDF NLNKKEYQNS SILVSFENFG SGSSREHAPW ALVDYGIRAI IAPSFADIFK NNALGNGLLT IELAKDEVLE IVDELKKSQD KNIEISLLEK RVFFKDKIFS FDLDDFHRIC LLEGLDNIAL TLKHEAQIKA YEKNSKSFLV  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAAAAATTTATTATACACAAAG  

PCR Backward Primer

TTATCCACTTCCAATGCTAAACCAAAAAGCTTTTTGAGT  

Shipped To Lab

ANL  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009