Clone

Target

IDP04265  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

208  

Reference Sequence Used

MKLTPMDINN KEFKRVLRGY CPEEVDEFLD QIVEEYEILF KENTALKEKL ENSNDKIEHY SKIENTIQNT LLLAQNTAEQ AKANAQKEAD VIIKSANDTA QRILDKAHND VIQINDEYDR VKQEFTKFRA KFRSFMNFQI DTFDDLEKDF IKNFNITKPE DQTETEEPQG IQIDFEEEKE EKTELKDIYE VETHFDDVNE IKSFFAEE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKLTPMDINN KEFKRVLRGY CPEEVDEFLD QIVEEYEILF KENTALKEKL ENSNDKIEHY SKIENTIQNT LLLAQNTAEQ AKANAQKEAD VIIKSANDTA QRILDKAHND VIQINDEYDR VKQEFTKFRA KFRSFMNFQI DTFDDLEKDF IKNFNITKPE DQTETEEPQG IQIDFEEEKE EKTELKDIYE VETHFDDVNE IKSFFAEE  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAATTAACCCCAATGGATAT  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCTTCAGCAAAGAAACTTT  

Shipped To Lab

ANL  

Shipped Date

Dec 29, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009