Clone

Target

IDP00002  

Clone Suffix

5

Sequence Comments

 

Experiment Start Date

May 28, 2010  

Experiment End Date

May 28, 2010  

Person

Hanna Tekleab  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

404  

Reference Sequence Used

MKKLIYLEEQ TMANKFLKTA TALTIMGTSL LGAGAFTVKA DNTDSLKFND VPANHWSTKA IYGLANRKVV AGYGNGQFGF GDNVTRGQVA RMIYAYVKPA DADASFKNPF SDIKGHMFEK EILALAKEGL VAGYGEGKFG PDDILTREQM AQVLTNAFKF KATKTTSFTD IDKNSWALKA ISALEENGVT IGTGDKLYSP YAHVTREQYS QFLFNSINVL EKETKPEVKP ETKPETKPET KPETKPETKP DVLDDKLVSN EFTFNKEWYE SSKVINNSSS IQAQSLINEV NKKYNTNLKY AEVGGVIRLV DNGMYLPEGS LKAQFYIVPI NEGSFKVIFL DNNDATVELA KKWTTLLKSD LNLDEEIQGT VESHKLNTFE KGNYKVRIGN STADHMMYVQ VESK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAAAACTAATATATTTGGAGG  

PCR Backward Primer

TTATCCACTTCCAATGCTACTTGCTCTCGACTTGAAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jul 26, 2010