Clone

Target

IDP00003  

Clone Suffix

5

Sequence Comments

 

Experiment Start Date

May 28, 2010  

Experiment End Date

May 28, 2010  

Person

Hanna Tekleab  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

317  

Reference Sequence Used

MKINRKGGLF LLMFLFLLTG CWDRRELEHV LFINTLGIDF KDNHYIIYPQ FINFTNMAKQ EGPANRNYSP AYIGKGEGKL LYDAAFDFYK SSQQKVSWEH IKSIVVTERF LKNGNLNQLN DFLSRFFQFR NTMWVFGTKE PLENILATNN IFNISSLYTI MNLPQETIRQ SASVDPLRLY TFRSNYYEPG MTTRIPFLAT TTEHWKQGHK AFWMLEFNEY GCIAAKSYVN HLNDSDIAGV RWTNKNTNRA PLLLKVKNRA IGELILKKPK IDTKVSIEKG KPHIVMNISL EGDIYQLMKP MPVHKVKKTL RISLKKK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKINRKGGLF LLMFLFLLTG CWDRRELEHV LFINTLGIDF KDNHYIIYPQ FINFTNMAKQ EGPANRNYSP AYIGKGEGKL LYDAAFDFYK SSQQKVSWEH IKSIVVTERF LKNGNLNQLN DFLSRFFQFR NTMWVFGTKE PLENILATNN IFNISSLYTI MNLPQETIRQ SASVDPLRLY TFRSNYYEPG MTTRIPFLAT TTEHWKQGHK AFWMLEFNEY GCIAAKSYVN HLNDSDIAGV RWTNKNTNRA PLLLKVKNRA IGELILKKPK IDTKVSIEKG KPHIVMNISL EGDIYQLMKP MPVHKVKKTL RISLKKK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAATAAATAGAAAAGGTGGG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTCTTTTTCAACGAGATCC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jul 26, 2010