Clone

Target
IDP05539  
Clone Suffix
1 The clone is not available in BEIR.
Experiment Start Date
Nov 06, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
250  
Reference Sequence Used
MNKVHFVGAG PGDKELITLK GYKLLSNADV VIYAGSLVNP ELLEYCKEDC QIHNSAHMDL QEIIDVMREG IENNKSVVRL QTGDFSIYGS IREQVEDLNK LNIDYDCTPG VSSFLGAASS LGVEYTVPEI SQSVIITRME GRTPVPEKES IQSYAKHQTS MVIFLSVQEI EKVVSKLLEG GYPKDTPIAV IYKATWADEK IVKGTLSDIA VKVKENNINK TALIMVGRFL GEEYNNSKLY DKDFKHEYRG  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MNKVHFVGAG PGDKELITLK GYKLLSNADV VIYAGSLVNP ELLEYCKEDC QIHNSAHMDL QEIIDVMREG IENNKSVVRL QTGDFSIYGS IREQVEDLNK LNIDYDCTPG VSSFLGAASS LGVEYTVPEI SQSVIITRME GRTPVPEKES IQSYAKHQTS MVIFLSVQEI EKVVSKLLEG GYPKDTPIAV IYKATWADEK IVKGTLSDIA VKVKENNINK TALIMVGRFL GEEYNNSKLY DKDFKHEYRG  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAATAAAGTACATTTTGTAGGA  
PCR Backward Primer
TTATCCACTTCCAATGTTACCCTCTATATTCATGTTTGA  
Shipped To Lab
ANL  
Shipped Date
Mar 03, 2011  
Inserted
Nov 24, 2010  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-05-24 2011-05-26 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production
2011-04-19 2011-04-21 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 0.5 mM IPTG production