Protocol

Abbreviation
allergens_purification_1
Name
just FPLC of purchased protein
Laboratory name
University of Virginia
Type
purification
Description
The protein is purchased from vendor (or obtained from natural source by collaborators).
The protein is dissolved in the buffer containing 10mM Tris-HCl pH 7.5 and 150mM NaCl.
After filtering the solution is passed through a Superdex 200 column attached to AKTA FPLC gel filtration system.